Those changes have given new impetus to forwards Gareth Bale, Karim Benzema and Marco Asensio to step up and fill the void left by Ronaldo, who scored 450 goals in 438 matches for Madrid.
Although an early move for Antoine Griezmann broke down when the striker chose to stay with Atletico Madrid, they quickly sealed deals for Sevilla centre-back Clement Lenglet, Gremio playmaker Arthur and Bordeaux winger Malcom.
Four Portuguese footballers, including Cristiano Ronaldo and Ricardinho, are among players up for UEFA's best Champions League forward.
Thomas Partey was part of the Atletico team that won the Europa League last season, and has been very present in the club's preseason.
Wednesday's matchup will provide Atletico with an opportunity to seize some momemtum prior to its La Liga opener on August 20. Find out by following our live matchcast. In the United States and Canada Eastern Time Zone that start time will be 3 p.m., noon in the Pacific time zone. The matches will be available on the UEFA Champions League Facebook Page.
Atletico, meanwhile, still have a 100% record in the UEFA Super Cup, having won it in 2010 and 2012.
"I know clearly who is going to play, but I will not give you the team", he said.
To watch the Real Madrid vs. Atletico Madrid UEFA Super Cup matchup live stream online from A. Le Coq Arena in Tallinn, Estonia, access the streaming video provided by Watch TNT, or by downloading the Watch TNT app to watch a live stream on mobile devices. Sign up now for a free seven-day trial.
Lopetegui, sacked by Spain on the eve of the World Cup after Madrid announced he would take over from Zidane, was reportedly not one of the club's top choices for the role.