The ministry said that the Canadian ambassador to Riyadh had 24 hours to leave the country, and announced the "freezing of all new commercial and investment transactions" with Ottawa.
Jackie Hansen, a gender rights campaigner for Amnesty International Canada, said last Thursday that the circumstances of the arrests were still unclear and there's no news of any charges.
"The kingdom of Saudi Arabia ... will not accept interference in its internal affairs or imposed diktats from any country", the foreign ministry tweeted.
The Saudis warned in a statement that any more criticism will be interpreted as licence to meddle in Canadian affairs.
"Any further step from the Canadian side in that direction will be considered as acknowledgment of our right to interfere in the Canadian domestic affairs", said the statement.
Canadian foreign ministry officials were not available for an immediate comment on Sunday.
Saudi authorities last week arrested two prominent women rights activists, Samar Badawi and Nassima al-Saadah, and held them incommunicado, Saudi human rights groups said. Freeland called on Saudi Arabia to release both. "Canada stands together with the Badawi family in this hard time, and we continue to strongly call for the release of both Raif and Samar Badawi".
On Friday, Canada called on Riyadh to "immediately release" civil and human rights activists recently arrested by the Saudi Arabian authorities, including Samar Badawi, an internationally recognized human rights defender.
Riyadh said Canada's statement about "civil society activists" was negative and unfair to the truth.
The ministry also affirmed that the Canadian position "is an explicit and clear interference in the internal affairs of the Kingdom of Saudi Arabia and contrary to the most basic global norms and all the charters that govern relations between countries". Saudi Arabia recalled its ambassador from Sweden and Stockholm cancelled a defence agreement with the Saudis.
An English version of the Saudi government's statement said Ms. Freeland and the Canadian government are wrong to criticize the kingdom.
It says the arrests were made by "the competent authorities" and the detainees were provided with all the rights guaranteed during investigative and trail stages.