"I hope they appear and tell everything about themselves", he said, addressing the audience during the Eastern Economic Forum (EEF) in the Russian city of Vladivostok.
"We know who these people are, we have found them", Mr Putin said.
Putin goes on to claim that his government has "found them" and he sincerely hopes that they'll come forward and tell their side of the story soon. Of his call for the men to speak publicly, Putin said, "It will be better for everyone".
"There is nothing special or criminal about it, I can assure you". We'll see in the near future, ' he added.
Asked about Putin's remarks, Prime Minister Theresa May's spokesman said Britain's attempts to get an explanation from Russian Federation over the poisoning of the Skripals had always been met with obfuscation and lies and that had not changed. The prime minister's spokesman said: 'These men used a devastatingly toxic illegal chemical weapon on the streets of our country.The government has exposed the role of the GRU and its operatives.
Prime Minister Theresa May said intelligence showed the poisonings were state-sponsored by Russian Federation. "I have seen nothing to suggest that has changed".
Russian Federation has repeatedly contested the allegations and on Wednesday Putin escalated the war of words by denying the men were members of military intelligence.
Annie Machon, a former MI5 intelligence officer, also doubted that the inquiry into the case has effectively turned into this trial by media, based on "bits of evidence that may look pretty compelling but will never be tested in a real court of law". Police posted detailed surveillance camera images tracking the two men moving through crowded British transport hubs, allegedly carrying the military-grade poison.
Petrov and Boshirov were being turned into Russian villains to the extent it was surprising they were not pictured in traditional Ushanka fur hats with ear flaps, she claimed.
Kremlin spokesman Dmitry Peskov said the president has not communicated with the men themselves since they were accused of poisoning the Skripals.
After the poisoning, Britain and more than two dozen other countries expelled a total of 150 Russian spies working under diplomatic cover. Russian news agency Fontanka has found traffic tickets and a Moscow apartment registered to Boshirov, but none of the neighbors know him.