O'Rourke made the comment Thursday night during a CNN town hall from the U.S. -Mexico border city of McAllen.
O'Rourke completely dodged the impeachment part of the question and talked about his vision of bringing Texans together regardless of their of political affiliations.
Asked if he regrets the comment, O'Rourke said: "I don't know that that's the way that I want to be talking in this campaign".
Pressed further by CNN's Dana Bash, O'Rourke said, "It's a definitive no".
"Have you changed your mind?" Ted Cruz's (R-Texas) Democrat Senate challenger won the final debate before the November 6 election. "But to quote George Will - very conservative columnist - when we saw him on that stage in Helsinki defending Vladimir Putin, the head of the country that attacked our democracy in 2016 instead of this country, and its citizens and this incredible democracy, that was collusion in action".
Morgan Ortagus said that O'Rourke, an El Paso lawmaker seeking to unseat Sen.
"The answer is no", he said when asked if he foresaw a future presidential bid.
O'Rourke said he "would not prejudge the outcome of that trial". She then cited the Constitution, saying impeachment means charging the president with having "committed the crime of treason, bribery, or high crime and misdemeanor".
"Umm, I'm not aware of any specific accomplishments", one student said, while another admitted, "I honestly couldn't point to anything".
"I hope they don't", Biden said told "CBS This Morning", before adding that his party should wait for the conclusions of the Russian Federation probe. "I'll now take that appreciation, '" Heidi Cruz noted, adding she "really hopes he wins his reelection".