County officials will appoint a Republican to take his place in the seat.
Officials are still waiting to determine Hof's cause of death.
Hof, 72, was found dead by porn actor Ron Jeremy on October 16 at the Love Ranch, one of the brothels Hof owned in Nevada.
Hof owned several brothels in Nevada, which is the only state that allows them to legally operate.
The pro-Trump candidate still found favour with the voters and patrons of Hof's Nevada brothel, even from the great beyond just to keep his memory alive.
Hof, who starred in the HBO reality television show Cathouse about the prostitutes working at one of his establishments, was running as a Donald Trump-style Republican.
The district is heavily rural, containing large desert areas in the southern region of Nevada.
Hof ran for office in 2016 as a Libertarian but lost the race. This summer, he ousted an incumbent Republican lawmaker in a primary election, celebrating at an election night party with "Hollywood Madam" Heidi Fleiss.
Hof, the self-described "Trump of Pahrump", was accused of rape and sexual assault by two of his former employees, according to the Review-Journal, citing a police report and interviews with the women.
Hof's campaign for District 36 - composed of Nye, Lincoln and Clark Counties - was marked by publicity stunts and billboard advertising that broadcast his pledges of repealing taxes and protecting gun rights, according to the US magazine The New Yorker.
Lt. David Boruchowitz with the Nye County Sheriff's Office confirmed Hof's death, but said the department is sharing no additional details.
The sprawling assembly district that touches both California and Utah and includes the Nevada National Security Site where nuclear weapons were once tested.