People block the traffic of Paris' landmark Avenue des Champs Elysees and place Charles de Gaulle-Etoile on November 17, 2018 in Paris.
Police arrested at least 117 protesters during the course of the demonstrations, and deployed riot police and tear gas in some areas to disperse protesters.
In the eastern Savoie region, a woman trying to get her daughter to a doctor panicked after protesters surrounded their auto and banged on the roof, accelerating into the crowd and killing a 63-year-old woman.
Eight of the 227 people injured were in serious condition, ministry officials said at an evening briefing, without providing details.
Wearing the safety hazard jackets drivers are mandated by law to carry in their auto, "Yellow Vests" are protesting the 23 per cent rise in diesel to €1.51 (£1.32/$1.71) per litre - the increase coming in a span of just 12 months and at its highest point since the early 2000s.
On Saturday a female protester was accidentally killed when a woman driver caught up in the blockade accelerated in panic and crashed into the crowd. The young woman appeared later on BFMTV still wearing her yellow vest.
The nationwide protest was unusual due to its grassroots origins. It was unclear whether the upstart movement, without a leader, would survive, and what problems it might pose for Macron. About 30 canisters of tear gas were fired to disperse protesters at the entrance of the Mont Blanc tunnel.
The amateur nature of the protests, often spontaneous and therefore illegal, made it tricky for police, who had orders to use dialogue instead of force but to stop protesters from completely blocking major routes.
Of the 409 injured, 28 are members of the police and fire brigade, said Christophe Castaner, the interior minister.
The government increased fuel taxes this year and will add another 6.5 cents per liter to diesel and 2.9 cents per liter to gasoline at the start of 2019, as it tries to bring diesel and gasoline taxes in line. A "carbon trajectory" calls for continued increases.
She said the price of diesel, for example, had risen 23% in the past 12 months.
Many drivers see this as emblematic of a presidency they view as disconnected from day-to-day economic difficulties and serving the rich.
The tax hike is so widely unpopular, that even 54 per cent of those who voted for Macron support the movement, with 73 per cent of Frenchmen of all political persuasions supporting the Yellow Vests.
"We've had enough of it". He also said more tax on fossil fuels was needed to fund renewable energy investments.
In Paris, several hundred protesters yelling "Macron resign!" and singing La Marseillaise massed on the Champs-Elysees and the Place de la Concorde, hoping to march toward the Elysee Palace, Macron's official residence.